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mouse igg1 anti human cd31 alexa fluor 647 mab  (Bio-Rad)


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    Structured Review

    Bio-Rad mouse igg1 anti human cd31 alexa fluor 647 mab
    Mouse Igg1 Anti Human Cd31 Alexa Fluor 647 Mab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+cd31/Mouse+anti+Human+CD31/pmc13691775-60-12-22
    Average 94 stars, based on 123 article reviews
    mouse igg1 anti human cd31 alexa fluor 647 mab - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Cell-Signaling:

    Article Title: Inhibition of immunoproteasome reduces infarction volume and attenuates inflammatory reaction in a rat model of ischemic stroke
    Article Snippet: For immunofluorescent assays, frozen sections (10 μ m) were prepared using a cryostat (Leica, CM1900) according to standard procedures. .. The following antibodies were used: rabbit anti-LMP2 (1 : 400; Abcam, Cambridge, UK), rabbit anti-LMP7 (1 : 300; Abcam), mouse anti-NeuN (1 : 400; Chemicon, Temecula, CA, USA), mouse anti-rat GFAP (1 : 800; Cell Signaling Technology, Beverly, MA, USA), mouse anti-rat OX-42 (1 : 300; Millipore, Billerica, MA, USA), mouse anti-CD31 (1 : 100; AbD Serotec, Kidlington, UK), mouse anti-CD4 (1 : 100; AbD Serotec), goat anti-rat-TNF-a (1 : 1000; R&D systems, Minneapolis, MN, USA), goat anti-rat-IL-1 β (1 : 1000; R&D Systems). .. Immunofluorescence was performed as described previously., Briefly, sections were pre-incubated with 0.3% Triton X-100 (v/v) in 0.01 M PBS (pH 7.4) for 10 min, followed by blocking in 10% normal goat serum (Kirkegaard & Perry Laboratories, Inc., Maryland, WA, USA) or 1% bovine serum albumin (MPBIO, Santa Ana, CA, USA) for 1 h at room temperature.

    Immunofluorescence:

    Article Title: Small-diameter bacterial cellulose-based vascular grafts for coronary artery bypass grafting in a pig model
    Article Snippet: .. For immunofluorescence staining, the following antibodies were used: rabbit anti-laminin (ab11575, dilution at 1:200) and goat anti-smooth muscle actin (SMA) (ab7817, dilution at 1:400) from Abcam (UK), goat anti-vascular endothelial (VE) cadherin (sc6458, dilution at 1:100) from Santa Cruz Biotechnology (Dallas, US), mouse anti-CD31 (MCA1746GA, dilution at 1:100) from AbD Serotec (Germany), mouse anti-CD68 (BA4D5, dilution at 1:50) from AbD Serotec (Germany). ..

    Staining:

    Article Title: Small-diameter bacterial cellulose-based vascular grafts for coronary artery bypass grafting in a pig model
    Article Snippet: .. For immunofluorescence staining, the following antibodies were used: rabbit anti-laminin (ab11575, dilution at 1:200) and goat anti-smooth muscle actin (SMA) (ab7817, dilution at 1:400) from Abcam (UK), goat anti-vascular endothelial (VE) cadherin (sc6458, dilution at 1:100) from Santa Cruz Biotechnology (Dallas, US), mouse anti-CD31 (MCA1746GA, dilution at 1:100) from AbD Serotec (Germany), mouse anti-CD68 (BA4D5, dilution at 1:50) from AbD Serotec (Germany). ..

    Immunohistochemistry:

    Article Title: Intracranial transplantation of monocyte-derived multipotential cells enhances recovery after ischemic stroke in rats.
    Article Snippet: Cell transplantation has emerged as a potential therapy to reduce the neurological deficits caused by ischemic stroke.. We previously reported a primitive cell population, monocyte-derived multipotential cells (MOMCs), which can differentiate into mesenchymal, neuronal, and endothelial lineages.. In this study, MOMCs and macrophages were prepared from rat peripheral blood and transplanted intracranially into the ischemic core of syngeneic rats that had undergone a left middle cerebral artery occlusion procedure.

    Incubation:

    Article Title: Intracranial transplantation of monocyte-derived multipotential cells enhances recovery after ischemic stroke in rats.
    Article Snippet: Cell transplantation has emerged as a potential therapy to reduce the neurological deficits caused by ischemic stroke.. We previously reported a primitive cell population, monocyte-derived multipotential cells (MOMCs), which can differentiate into mesenchymal, neuronal, and endothelial lineages.. In this study, MOMCs and macrophages were prepared from rat peripheral blood and transplanted intracranially into the ischemic core of syngeneic rats that had undergone a left middle cerebral artery occlusion procedure.

    Article Title: An implantable human stem cell-derived tissue-engineered rostral migratory stream for directed neuronal replacement
    Article Snippet: Subsequently, cultures were rinsed and incubated in appropriate Alexa secondary antibodies (1:500) in the dark at 37 °C for 2 h. Rat cortical astrocyte planar cultures, extracted rat cortical astrocyte TE-RMSs, human gingiva stem cell-derived planar cultures, and extracted human gingiva stem cell-derived TE-RMSs were incubated in mouse anti-Ezrin (1:100) (Sigma-Aldrich Cat # E8897, RRID: AB_476955), goat anti-GFAP (1:1000) (Abcam Cat # ab53554, RRID: AB_880202), and rabbit anti-Robo2 (1:50) (Novus Cat # NBP1-81399, RRID: AB_11013687) followed by secondary antibodies donkey anti-mouse 488 (Thermo Fisher Scientific Cat#: A-21202, RRID: AB_141607), donkey anti-goat 568 (Thermo Fisher Scientific Cat#: A-11057, RRID: AB_2534104), and donkey anti-rabbit 647 (Thermo Fisher Scientific Cat#: A-31573, RRID: AB_2536183). .. To verify astrocytic phenotype, human gingiva stem cell-derived astrocyte-like planar cultures were incubated in mouse anti-CD31 (1:100) (Bio-Rad Cat#: MCA1746GA, RRID: AB_2832958) to label endothelial cells, and guinea pig anti-S100B (1:200) (Synaptic systems Cat #: 287 004, RRID: AB_2620025), chicken anti-GFAP (1:1000) (Abcam Cat #: ab4674, RRID: AB_304558), rabbit anti-GLAST ( EAAT1 ) (Abcam Cat #: ab41751, RRID: AB_955879), and mouse anti-glutamine synthetase (Abcam Cat #: ab64613, RRID: AB_1140869) to label astrocytes; secondary antibodies were donkey anti-mouse 488 (Thermo Fisher Scientific Cat#: A-21202, RRID: AB_141607), donkey anti-guinea pig 568 (Sigma Cat#: SAB4600469, RRID: AB_2832959), donkey anti-rabbit 568 (Thermo Fisher Scientific Cat #: A10042, RRID: AB_2534017), and donkey anti-chicken 647 (Jackson Immunoresearch Cat#: 703-605-155, RRID: AB_2340379). .. Fixed human GMSC-derived TE-RMSs loaded with cortical neuronal aggregates were incubated in mouse anti-human nuclei (1:200) (Millipore Cat #: MAB1281, RRID: AB_94090) to label human astrocytes, rabbit anti-beta III tubulin (TuJ1) (1:500) (Abcam Cat#: ab18207, RRID: AB_444319) to label immature migrating neurons, and goat anti-GFAP (Abcam Cat # ab53554, RRID: AB_880202)) to label astrocytes in TE-RMSs; followed by secondary antibodies donkey anti-mouse 488 (Thermo Fisher Scientific Cat#: A-21202, RRID: AB_141607), donkey anti-rabbit 568 (Thermo Fisher Scientific Cat #: A10042, RRID: AB_2534017), and donkey anti-goat 647 (Thermo Fisher Scientific Cat# A-21447, RRID: AB_2535864).

    Article Title: Guanylate-Binding Protein-1 Expression Is Selectively Induced by Inflammatory Cytokines and Is an Activation Marker of Endothelial Cells during Inflammatory Diseases
    Article Snippet: .. After antigen retrieval as described above, the slides were blocked with 10% normal goat serum for 10 minutes at room temperature and subsequently incubated overnight at 4°C with a mixture of mAb 1B1 (1:100) and mouse anti-CD31 (1:20; Serotec, Oxford, UK). ..

    Article Title: lncRNA H19 prevents endothelial-mesenchymal transition in diabetic retinopathy.
    Article Snippet: A bicinchoninic acid protein assay kit (Pierce, Rockford, IL, USA) was used to determine protein concentration. .. A total of 30μg of protein was resolved by 10% SDS gel electrophoresis, transferred to a polyvinylidene difluoride membrane (Bio-Rad, Hercules, CA, USA) and incubated overnight at 4°C with one of the primary antibodies: mouse anti-CD31, rabbit anti-FSP1 (1:1000 and 1:100, respectively; Abcam); rabbit anti-SM22, mouse anti-β-actin antibody (1:200 and 1:400, respectively; Santa Cruz); rabbit anti-p-ERK1/2, rabbit anti-ERK1/2, rabbit anti-p-Smad2/3, mouse anti-Smad2/3 (1:1000; Cell Signaling Technology, Beverly, MA, USA); polyclonal anti-Akt and anti-p-Akt (Ser473 and Thr308) (1:1000; New England BioLabs, Pickering, ON, Canada). .. The membranes were then incubated with HRP-conjugated anti-rabbit antibody (1:10,000; Upstate Biotechnology, Charlottesville, VA, USA) or anti-mouse antibody (1:10,000; Vector Laboratories) for 1 h at room temperature [36].

    SDS-Gel:

    Article Title: lncRNA H19 prevents endothelial-mesenchymal transition in diabetic retinopathy.
    Article Snippet: A bicinchoninic acid protein assay kit (Pierce, Rockford, IL, USA) was used to determine protein concentration. .. A total of 30μg of protein was resolved by 10% SDS gel electrophoresis, transferred to a polyvinylidene difluoride membrane (Bio-Rad, Hercules, CA, USA) and incubated overnight at 4°C with one of the primary antibodies: mouse anti-CD31, rabbit anti-FSP1 (1:1000 and 1:100, respectively; Abcam); rabbit anti-SM22, mouse anti-β-actin antibody (1:200 and 1:400, respectively; Santa Cruz); rabbit anti-p-ERK1/2, rabbit anti-ERK1/2, rabbit anti-p-Smad2/3, mouse anti-Smad2/3 (1:1000; Cell Signaling Technology, Beverly, MA, USA); polyclonal anti-Akt and anti-p-Akt (Ser473 and Thr308) (1:1000; New England BioLabs, Pickering, ON, Canada). .. The membranes were then incubated with HRP-conjugated anti-rabbit antibody (1:10,000; Upstate Biotechnology, Charlottesville, VA, USA) or anti-mouse antibody (1:10,000; Vector Laboratories) for 1 h at room temperature [36].

    Electrophoresis:

    Article Title: lncRNA H19 prevents endothelial-mesenchymal transition in diabetic retinopathy.
    Article Snippet: A bicinchoninic acid protein assay kit (Pierce, Rockford, IL, USA) was used to determine protein concentration. .. A total of 30μg of protein was resolved by 10% SDS gel electrophoresis, transferred to a polyvinylidene difluoride membrane (Bio-Rad, Hercules, CA, USA) and incubated overnight at 4°C with one of the primary antibodies: mouse anti-CD31, rabbit anti-FSP1 (1:1000 and 1:100, respectively; Abcam); rabbit anti-SM22, mouse anti-β-actin antibody (1:200 and 1:400, respectively; Santa Cruz); rabbit anti-p-ERK1/2, rabbit anti-ERK1/2, rabbit anti-p-Smad2/3, mouse anti-Smad2/3 (1:1000; Cell Signaling Technology, Beverly, MA, USA); polyclonal anti-Akt and anti-p-Akt (Ser473 and Thr308) (1:1000; New England BioLabs, Pickering, ON, Canada). .. The membranes were then incubated with HRP-conjugated anti-rabbit antibody (1:10,000; Upstate Biotechnology, Charlottesville, VA, USA) or anti-mouse antibody (1:10,000; Vector Laboratories) for 1 h at room temperature [36].

    Membrane:

    Article Title: lncRNA H19 prevents endothelial-mesenchymal transition in diabetic retinopathy.
    Article Snippet: A bicinchoninic acid protein assay kit (Pierce, Rockford, IL, USA) was used to determine protein concentration. .. A total of 30μg of protein was resolved by 10% SDS gel electrophoresis, transferred to a polyvinylidene difluoride membrane (Bio-Rad, Hercules, CA, USA) and incubated overnight at 4°C with one of the primary antibodies: mouse anti-CD31, rabbit anti-FSP1 (1:1000 and 1:100, respectively; Abcam); rabbit anti-SM22, mouse anti-β-actin antibody (1:200 and 1:400, respectively; Santa Cruz); rabbit anti-p-ERK1/2, rabbit anti-ERK1/2, rabbit anti-p-Smad2/3, mouse anti-Smad2/3 (1:1000; Cell Signaling Technology, Beverly, MA, USA); polyclonal anti-Akt and anti-p-Akt (Ser473 and Thr308) (1:1000; New England BioLabs, Pickering, ON, Canada). .. The membranes were then incubated with HRP-conjugated anti-rabbit antibody (1:10,000; Upstate Biotechnology, Charlottesville, VA, USA) or anti-mouse antibody (1:10,000; Vector Laboratories) for 1 h at room temperature [36].



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    ( A ) Representative parasternal long-axis views and M-mode images. ( B ) Echocardiographic analysis of EF, FS, LVESV, LVEDV, LVESD, and LVEDD on days 0, 7, and 14 after MI or sham operation in Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 8). ( C ) Masson’s trichrome staining for infarct size measurements in Sik2 F/F and Sik2 F/F ; LysM Cre mice at day 14 after MI or sham operation ( n = 8). ( D ) Post-MI survival analysis. n = 42 in Sik2 F/F MI; n = 38 in Sik2 F/F ; LysM Cre MI. ( E ) Masson’s trichrome and Picrosirius red (PSR) staining of heart transverse sections to detect collagen within the scar of Sik2 F/F and Sik2 F/F ; LysM Cre hearts at day 14 post-MI (scale bar, 100 μm). Collagen density measured as the total collagen area divided by scar area examined ( n = 7). ( F ) Immunostaining analyses of α-SMA (green), Collagen I (green) and DAPI (blue) in the heart tissue at day 7 post-MI ( n = 8) (scale bar, 40 μm). ROI indicates the region of interest. ( G ) Gating strategy for endothelial cells (ECs) and CFs in the infarcted heart. ( H ) Statistics of ECs and CFs in the infarcted heart at day 7 post-MI from Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 6). ( I ) Representative fibroblasts immunostaining of α-SMA (green), Collagen I (red), and DAPI (blue). Scale bar, 20 μm.
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    ( A ) Representative parasternal long-axis views and M-mode images. ( B ) Echocardiographic analysis of EF, FS, LVESV, LVEDV, LVESD, and LVEDD on days 0, 7, and 14 after MI or sham operation in Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 8). ( C ) Masson’s trichrome staining for infarct size measurements in Sik2 F/F and Sik2 F/F ; LysM Cre mice at day 14 after MI or sham operation ( n = 8). ( D ) Post-MI survival analysis. n = 42 in Sik2 F/F MI; n = 38 in Sik2 F/F ; LysM Cre MI. ( E ) Masson’s trichrome and Picrosirius red (PSR) staining of heart transverse sections to detect collagen within the scar of Sik2 F/F and Sik2 F/F ; LysM Cre hearts at day 14 post-MI (scale bar, 100 μm). Collagen density measured as the total collagen area divided by scar area examined ( n = 7). ( F ) Immunostaining analyses of α-SMA (green), Collagen I (green) and DAPI (blue) in the heart tissue at day 7 post-MI ( n = 8) (scale bar, 40 μm). ROI indicates the region of interest. ( G ) Gating strategy for endothelial cells (ECs) and CFs in the infarcted heart. ( H ) Statistics of ECs and CFs in the infarcted heart at day 7 post-MI from Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 6). ( I ) Representative fibroblasts immunostaining of α-SMA (green), Collagen I (red), and DAPI (blue). Scale bar, 20 μm.
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    Image Search Results


    ( A ) Representative parasternal long-axis views and M-mode images. ( B ) Echocardiographic analysis of EF, FS, LVESV, LVEDV, LVESD, and LVEDD on days 0, 7, and 14 after MI or sham operation in Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 8). ( C ) Masson’s trichrome staining for infarct size measurements in Sik2 F/F and Sik2 F/F ; LysM Cre mice at day 14 after MI or sham operation ( n = 8). ( D ) Post-MI survival analysis. n = 42 in Sik2 F/F MI; n = 38 in Sik2 F/F ; LysM Cre MI. ( E ) Masson’s trichrome and Picrosirius red (PSR) staining of heart transverse sections to detect collagen within the scar of Sik2 F/F and Sik2 F/F ; LysM Cre hearts at day 14 post-MI (scale bar, 100 μm). Collagen density measured as the total collagen area divided by scar area examined ( n = 7). ( F ) Immunostaining analyses of α-SMA (green), Collagen I (green) and DAPI (blue) in the heart tissue at day 7 post-MI ( n = 8) (scale bar, 40 μm). ROI indicates the region of interest. ( G ) Gating strategy for endothelial cells (ECs) and CFs in the infarcted heart. ( H ) Statistics of ECs and CFs in the infarcted heart at day 7 post-MI from Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 6). ( I ) Representative fibroblasts immunostaining of α-SMA (green), Collagen I (red), and DAPI (blue). Scale bar, 20 μm.

    Journal: Science Advances

    Article Title: SIK2 antagonizes postinfarction cardiac repair by driving macrophage proinflammatory and impairing efferocytosis

    doi: 10.1126/sciadv.adw6624

    Figure Lengend Snippet: ( A ) Representative parasternal long-axis views and M-mode images. ( B ) Echocardiographic analysis of EF, FS, LVESV, LVEDV, LVESD, and LVEDD on days 0, 7, and 14 after MI or sham operation in Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 8). ( C ) Masson’s trichrome staining for infarct size measurements in Sik2 F/F and Sik2 F/F ; LysM Cre mice at day 14 after MI or sham operation ( n = 8). ( D ) Post-MI survival analysis. n = 42 in Sik2 F/F MI; n = 38 in Sik2 F/F ; LysM Cre MI. ( E ) Masson’s trichrome and Picrosirius red (PSR) staining of heart transverse sections to detect collagen within the scar of Sik2 F/F and Sik2 F/F ; LysM Cre hearts at day 14 post-MI (scale bar, 100 μm). Collagen density measured as the total collagen area divided by scar area examined ( n = 7). ( F ) Immunostaining analyses of α-SMA (green), Collagen I (green) and DAPI (blue) in the heart tissue at day 7 post-MI ( n = 8) (scale bar, 40 μm). ROI indicates the region of interest. ( G ) Gating strategy for endothelial cells (ECs) and CFs in the infarcted heart. ( H ) Statistics of ECs and CFs in the infarcted heart at day 7 post-MI from Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 6). ( I ) Representative fibroblasts immunostaining of α-SMA (green), Collagen I (red), and DAPI (blue). Scale bar, 20 μm.

    Article Snippet: Primary antibodies used to specifically detect the proteins of interest were as follows: SIK2 (6919s, CST), GAPDH (G9545, Sigma-Aldrich), α-SMA (A5228, Sigma-Aldrich), Collagen I (AB765P, Sigma-Aldrich), Collagen III (AB7778, Abcam), CD31 (3528s, CST), Phospho-NF-κB P65 (Ser 536 ) (3033s, CST), NF-κB P65 (8242s, CST), Phospho-P38 MAPK (Thr 180 /Tyr 182 ) (4511s, CST), P38 MAPK (8690s, CST), α-Tubulin (66031-1, Proteintech), Histone H3 (68345-1, Proteintech), NOS2 (13120s, CST), FLAG-Tag (14793s, CST), HA-Tag (3724s, CST), TRAF1 (4710s, CST), TRAF2 (4724s, CST), TRAF4 (18527s, CST), TRAF6 (67591s, CST), His-Tag (12698s, CST), and Myc-Tag (2276s, CST).

    Techniques: Analysis, Staining, Immunostaining, Tissue